2 edition of Regulation of sRNA synthesis in Escherichia coli. found in the catalog.
Regulation of sRNA synthesis in Escherichia coli.
Merrilyn Dawn Mustard
Written in English
|Contributions||Toronto, Ont. University. Theses (M.Sc.)|
|LC Classifications||LE3 T525 MSC 1967 M88|
|The Physical Object|
|Number of Pages||46|
Abstract. It is now clear that in Escherichia coli the synthesis of many of the components involved in transcription and translation are carefully regulated. For example, the expression of a number of these genes is under stringent control (Dennis and Nomura, ; Furano and Wittel, ), the mechanism by which bacteria, when starved for an amino acid, turn off the synthesis of ribosomal RNA. INTRODUCTION. Since the identification of the structural gene for Escherichia coli poly(A) polymerase I (PAP I) in (), a variety of experiments have shown that polyadenylation plays an integral role in RNA metabolism (2– 7).Specifically, the deletion of the structural gene for PAP I (pcnB) leads to a 90% reduction in poly(A) levels along with increased mRNA half-lives (2, 5).
ModE is the molybdate-sensing transcription regulator that controls the expression of genes related to molybdate homeostasis in Escherichia coli. ModE is activated by binding molybdate and acts as both an activator and a repressor. By genomic systematic evolution of ligands by exponential enrichment (SELEX) screening and promoter reporter assays, we have identified a total of nine operons. Nitrogen limitation in Escherichia coli controls the expression of about genes of the nitrogen regulated (Ntr) response, including the ammonia-assimilating glutamine synthetase. Low intracellular glutamine controls the Ntr response through several regulators, whose activities are modulated by a .
The stationary-phase sigma factor (RpoS) regulates many cellular responses to environmental stress conditions such as heat, acid, and alkali shocks. On the other hand, mutations at the rpoS locus have frequently been detected among pathogenic as well as commensal strains of Escherichia coli. The objective of this study was to perform a functional analysis of the RpoS-mediated stress responses. Introduction. Entry of Escherichia coli cells into stationary phase results in complex morphological and physiological changes (Siegele and Kolter, ; Hengge‐Aronis et al., ).Stationary phase cells become more resistant to a variety of stresses including starvation, near UV radiation, high temperature, hydrogen peroxide, acidic pH and high medium osmolarity (Loewen and Hengge.
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Bremer H, Berry L, Dennis PP. Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. Fraction of RNA polymerase engaged in the synthesis of stable RNA at different steady-state growth rates.
J Mol Biol. Mar 25; 75 (1)– Taylor AL, Trotter CD. Linkage map of Escherichia coli strain KCited by: Regulation of pyrBI operon expression in Escherichia coli by UTP-sensitive reiterative RNA synthesis during transcriptional initiation.
C Liu, L S Heath, and C L Turnbough; Department of Microbiology, University of Alabama at Birmingham Cited by: These experiments investigate two aspects of RNA synthesis in Escherichia coli ML30 during the transition from a relatively slow rate of growth to a more rapid one: (1) the number of growing RNA molecules per cell, and (2) the average time required for addition of a nucleotide onto a growing RNA chain.
Cells were grown at 30°C in a glucose-minimal salts medium and shifted-up by the addition Cited by: 4. (This subject is extensively reviewed in refs. 1–4.) Thus at high growth rates ribosomal RNA is accumulated rapidly, the synthesis of ribosomal RNA accounting for up to 40% of the instantaneous rate of RNA synthesis (5,6), although the cistrons coding for these RNA species comprise less than % of the bacterial genome (7,8).Cited by: 2.
Journals & Books; Register Sign in. Vol Issue 2, 28 OctoberPages Regulation of messenger RNA synthesis in Escherichia coli Quantitative estimates of messenger activity have been obtained using RNA extracted from Escherichia coli to stimulate in vitro Cited by: Under the balanced condition of growth of E.
coli cells, no distinct difference is observed in stable RNA and protein synthesis between CP78 (rel +) and CP79 (rel −), whereas a considerable difference is present in RNA accumulation between NF (rel +) and NF (rel −), where NF RNA content of NF is lower than that of NF in four different cultures with different Cited by: THE ribosomal cistrons of Escherichia coli comprise only to % of the genome and yet in exponentially growing cultures 40% of the instantaneous rate of RNA synthesis.
A temperature shift-up accompanied by a reduction in RNA polymerase activity in Escherichia coli causes an increased rate of initiation leading to a to fold increase in chromosome copy number. A temperature shift-up without a reduction in polymerase activity induces only a transient non-scheduled initiation of chromosome replication caused by heat shock with no detectable effect on.
Abstract. Transcription initiation is accompanied with iterative synthesis and release of short transcripts. The molar ratio of enzyme to template was found to be critical for the amounts and distribution of the abortive products synthesized by Escherichia coli RNA polymerase from several promoters.
At a high ratio abortive synthesis of 4–8 nt were enhanced at the γ P R promoter. The Escherichia coli Crl protein has been described as a transcriptional coactivator for the stationary-phase sigma factor σS.
In a transcription system with highly purified components, we demonstrate that Crl affects transcription not only by the EσS RNA polymerase holoenzyme but also by Eσ70 and Eσ Crl increased transcription dramatically but only when the σ concentration was low and.
Buxton RS. Genetic analysis of Escherichia coli K12 mutants resistant to bacteriophage BF23 and the E-group colicins. Mol Gen Genet. ; (2)– Austin S, McGeoch D.
The synthesis in vitro of RNA polymerase subunits of Escherichia coli. Proc Natl Acad Sci U S A. Aug; 70 (8)– [PMC free article] Austin S, Scaife J.
Variations in serine transhydroxymethylase (STHM) activity as a function of the addition of certain nutrilites to the growth medium of cells of Escherichia coli K12 have been studied. STHM activity was decreased by growth in (a) glycine at high concentrations, and (b) a mixture of C 1-derived metabolites (histidine, methionine, adenine, guanine, thymidine) at moderate concentrations.
Kumar R, Shimizu K: Metabolic regulation of Escherichia coli and its gdhA, glnL, gltB, D mutants under different carbon and nitrogen limitations in the continuous culture. Microbial Cell Factories.9: 8- / Bacterial cell-to-cell communication facilitates coordinated expression of specific genes in a growth rate-II and cell density-dependent manner, a process known as quorum sensing.
While the discovery of a diffusible Escherichia coli signaling pheromone, termed autoinducer 2 (AI-2), has been made along with several quorum sensing genes, the overall number and coordination of genes controlled by. Chatterji D, Fujita N, Ishihama A: The mediator for stringent control, ppGpp, binds to the beta-subunit of Escherichia coli RNA polymerase.
Genes to Cells. 3: /jx. Article CAS PubMed Google Scholar. The effect of antibiotics which prevent, either directly or indirectly, the formation of polyribosomes (i.e. the translocation of ribosomes along messenger RNA), including fusidic acid, erythromycin, tetracycline, chloramphenicol and puromycin, on the synthesis of trp messenger RNA and bulk messenger RNA in Escherichia coli was studied.
Quantitative estimates of the synthesis of messenger RNA. Dennis PP, Nomura M. Regulation of the expression of ribosomal protein genes in Escherichia coli. J Mol Biol. Sep 5; 97 (1)– Dennis PP, Nomura M. Stringent control of ribosomal protein gene expression in Escherichia coli.
Proc Natl Acad Sci U S A. PHOSPHORUS STARVATION. coli cells also enter stationary phase upon phosphorus limitation. Under these conditions, RpoS levels increase to a degree similar to that seen with carbon starvation, and RpoS-dependent gene expression follows accordingly (13, 34, 39).This occurs despite continuing slow growth, which we suspect results from the mobilization of intracellular polyphosphate stores.
Jishage M, Ishihama A. Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of sigma 70 and sigma J Bacteriol. Cashel M, Gallant J. Control of RNA synthesis in Escherichia coli.
Amino acid dependence of the synthesis of the substrates of RNA polymerase. J Mol Biol. Jul 14; 34 (2)– Edlin G, Neuhard J. Regulation of nucleoside triphosphate pools in Escherichia coli. J Mol Biol. Mar 14; 24 (2)– News in brief.
Science. The rate of synthesis of the β and β′ subunits of RNA polymerase relative to the rate of synthesis of total protein was found to remain constant with increasing steady state growth rate.
This is in contrast to the relative synthesis rates of ribosomal proteins which are known to increase with growth rate. Yet the ratio of the rate of transcription of the ribosomal protein (rplJL) and RNA. Ribonuclease III of the gram‐negative bacterium Escherichia coli processes rRNA and mRNA precursors, and its catalytic action can regulate gene expression by controlling mRNA translation and stability.
It has been proposed that RNase III can function in a non‐catalytic manner, by binding RNA without cleaving phosphodiesters. However.A RNA-DNA hybridization assay for ribonucleoside diphosphate reductase (RDP reductase) mRNA was used to determine whether control of RDP reductase synthesis in Escherichia coli is at the level of.